BAIT

GAL80

transcription regulator GAL80, L000000665, YML051W

Transcriptional regulator involved in the repression of GAL genes; involved in the repression of GAL genes in the absence of galactose; inhibits transcriptional activation by Gal4p; inhibition relieved by Gal3p or Gal1p binding

GO Process (4)

GO Function (2)

GO Component (2)

Gene Ontology Biological Process

galactose metabolic process [IMP]

negative regulation of kinase activity [IDA]

negative regulation of sequence-specific DNA binding transcription factor activity [IDA, IPI]

negative regulation of transcription from RNA polymerase II promoter [IMP]

Gene Ontology Molecular Function

RNA polymerase II activating transcription factor binding [IDA, IPI]
kinase inhibitor activity [IDA]

Gene Ontology Cellular Component

cytoplasm [IDA]

nucleus [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

HAP3

L000000753, YBL021C

Subunit of the Hap2p/3p/4p/5p CCAAT-binding complex; complex is heme-activated and glucose-repressed; complex is a transcriptional activator and global regulator of respiratory gene expression; contains sequences contributing to both complex assembly and DNA binding

GO Process (2)

GO Function (2)

GO Component (1)

Gene Ontology Biological Process

carbon catabolite activation of transcription from RNA polymerase II promoter [IDA]

regulation of cellular respiration [IMP]

Gene Ontology Molecular Function

RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]

Gene Ontology Cellular Component

CCAAT-binding factor complex [IDA, IPI]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

Epistatic relationships reveal the functional organization of yeast transcription factors.

Zheng J, Benschop JJ, Shales M, Kemmeren P, Greenblatt J, Cagney G, Holstege F, Li H, Krogan NJ

The regulation of gene expression is, in large part, mediated by interplay between the general transcription factors (GTFs) that function to bring about the expression of many genes and site-specific DNA-binding transcription factors (STFs). Here, quantitative genetic profiling using the epistatic miniarray profile (E-MAP) approach allowed us to measure 48 391 pairwise genetic interactions, both negative (aggravating) and positive (alleviating), ... [more]

Mol. Syst. Biol. Oct. 05, 2010; 6(0);420 [Pubmed: 20959818]

Quantitative Score

-2.814517267 [SGA Score]

Throughput

High Throughput

Ontology Terms

phenotype: colony size (APO:0000063)

Additional Notes

An Epistatic MiniArray Profile (E-MAP) approach was used to quantitatively score genetic interactions based on fitness defects estimated from the colony size of double versus single mutants. Genetic interactions were considered significant if they had an S score > 2.5 for positive interactions (epistatic or suppressor interactions) and S score < -2.5 for negative interactions (synthetic sick/lethal interactions).

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
HAP3 GAL80	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2010)	High	-0.1858	BioGRID	356676
HAP3 GAL80	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2016)	High	-0.1416	BioGRID	2077649

Curated By

BioGRID

Interaction Summary

GAL80

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase II activating transcription factor binding [IDA, IPI]kinase inhibitor activity [IDA]

Gene Ontology Cellular Component

HAP3

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]

Gene Ontology Cellular Component

Negative Genetic

Publication

Epistatic relationships reveal the functional organization of yeast transcription factors.

Quantitative Score

Throughput

Ontology Terms

Additional Notes

Related interactions

Curated By

RNA polymerase II activating transcription factor binding [IDA, IPI]
kinase inhibitor activity [IDA]

RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]