BAIT
ELF1
YKL160W
Transcription elongation factor with a conserved zinc finger domain; implicated in the maintenance of proper chromatin structure in actively transcribed regions; deletion inhibits Brome mosaic virus (BMV) gene expression
GO Process (2)
GO Function (1)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
CRZ1
HAL8, TCN1, DNA-binding transcription factor CRZ1, L000004202, YNL027W
Transcription factor, activates transcription of stress response genes; nuclear localization is positively regulated by calcineurin-mediated dephosphorylation; rapidly localizes to the nucleus under blue light stress; can be activated in stochastic pulses of nuclear localization in response to calcium
GO Process (12)
GO Function (3)
GO Component (2)
Gene Ontology Biological Process
- cell wall chitin biosynthetic process [IGI]
- cellular response to blue light [IDA]
- ion homeostasis [IGI, IMP]
- positive regulation of cellular response to drug [IMP]
- positive regulation of sodium ion transport by positive regulation of transcription from RNA polymerase II promoter [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IGI, IMP]
- positive regulation of transcription from RNA polymerase II promoter by calcium-mediated signaling [IGI, IMP]
- positive regulation of transcription from RNA polymerase II promoter by pheromones [IMP]
- positive regulation of transcription from RNA polymerase II promoter in response to alkaline pH [IMP]
- positive regulation of transcription from RNA polymerase II promoter in response to calcium ion [IMP]
- positive regulation of transcription from RNA polymerase II promoter in response to increased salt [IMP]
- regulation of potassium ion concentration by positive regulation of transcription from RNA polymerase II promoter [IDA, IGI, IMP]
Gene Ontology Molecular Function
Saccharomyces cerevisiae (S288c)
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Epistatic relationships reveal the functional organization of yeast transcription factors.
The regulation of gene expression is, in large part, mediated by interplay between the general transcription factors (GTFs) that function to bring about the expression of many genes and site-specific DNA-binding transcription factors (STFs). Here, quantitative genetic profiling using the epistatic miniarray profile (E-MAP) approach allowed us to measure 48 391 pairwise genetic interactions, both negative (aggravating) and positive (alleviating), ... [more]
Mol. Syst. Biol. Oct. 05, 2010; 6(0);420 [Pubmed: 20959818]
Quantitative Score
- -3.376203225 [SGA Score]
Throughput
- High Throughput
Ontology Terms
- phenotype: colony size (APO:0000063)
Additional Notes
- An Epistatic MiniArray Profile (E-MAP) approach was used to quantitatively score genetic interactions based on fitness defects estimated from the colony size of double versus single mutants. Genetic interactions were considered significant if they had an S score > 2.5 for positive interactions (epistatic or suppressor interactions) and S score < -2.5 for negative interactions (synthetic sick/lethal interactions).
Curated By
- BioGRID