BAIT
MET32
L000003470, YDR253C
Zinc-finger DNA-binding transcription factor; involved in transcriptional regulation of the methionine biosynthetic genes; targets strong transcriptional activator Met4p to promoters of sulfur metabolic genes; feedforward loop exists in the regulation of genes controlled by Met4p and Met32p; lack of such a loop for MET31 may account for the differential actions of Met32p and Met31p; MET32 has a paralog, MET31, that arose from the whole genome duplication
GO Process (5)
GO Function (4)
GO Component (2)
Gene Ontology Biological Process
- negative regulation of transcription from RNA polymerase II promoter [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IMP]
- regulation of mitotic cell cycle [IGI, IMP]
- regulation of sulfur amino acid metabolic process [IDA, IGI, IMP]
- regulation of transcription from RNA polymerase II promoter [IDA, IGI]
Gene Ontology Molecular Function- RNA polymerase II activating transcription factor binding [IDA, IPI]
- core promoter proximal region sequence-specific DNA binding [IDA]
- sequence-specific DNA binding [IDA]
- sequence-specific transcription regulatory region DNA binding RNA polymerase II transcription factor recruiting transcription factor activity [IDA, IGI, IPI]
- RNA polymerase II activating transcription factor binding [IDA, IPI]
- core promoter proximal region sequence-specific DNA binding [IDA]
- sequence-specific DNA binding [IDA]
- sequence-specific transcription regulatory region DNA binding RNA polymerase II transcription factor recruiting transcription factor activity [IDA, IGI, IPI]
Saccharomyces cerevisiae (S288c)
PREY
URE2
[URE3], L000002439, YNL229C
Nitrogen catabolite repression transcriptional regulator; inhibits GLN3 transcription in good nitrogen source; role in sequestering Gln3p and Gat1p to the cytoplasm; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion
GO Process (5)
GO Function (3)
GO Component (1)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Saccharomyces cerevisiae (S288c)
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Epistatic relationships reveal the functional organization of yeast transcription factors.
The regulation of gene expression is, in large part, mediated by interplay between the general transcription factors (GTFs) that function to bring about the expression of many genes and site-specific DNA-binding transcription factors (STFs). Here, quantitative genetic profiling using the epistatic miniarray profile (E-MAP) approach allowed us to measure 48 391 pairwise genetic interactions, both negative (aggravating) and positive (alleviating), ... [more]
Mol. Syst. Biol. Oct. 05, 2010; 6(0);420 [Pubmed: 20959818]
Quantitative Score
- -2.521328781 [SGA Score]
Throughput
- High Throughput
Ontology Terms
- phenotype: colony size (APO:0000063)
Additional Notes
- An Epistatic MiniArray Profile (E-MAP) approach was used to quantitatively score genetic interactions based on fitness defects estimated from the colony size of double versus single mutants. Genetic interactions were considered significant if they had an S score > 2.5 for positive interactions (epistatic or suppressor interactions) and S score < -2.5 for negative interactions (synthetic sick/lethal interactions).
Curated By
- BioGRID