AFT1
Gene Ontology Biological Process
- chromosome segregation [IMP]
- establishment of mitotic sister chromatid cohesion [IMP]
- meiotic chromosome segregation [IMP]
- positive regulation of iron ion transport [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- positive regulation of transcription from RNA polymerase II promoter in response to iron ion starvation [IMP]
Gene Ontology Molecular Function
CBF1
Gene Ontology Biological Process
- chromatin remodeling [IDA, IMP]
- chromosome segregation [IGI, IMP]
- negative regulation of ceramide biosynthetic process by negative regulation of transcription from RNA Polymerase II promoter [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IMP]
- positive regulation of inositol biosynthetic process by positive regulation of transcription from RNA polymerase II promoter [IMP]
- positive regulation of sulfate assimilation by positive regulation of transcription from RNA polymerase II promoter [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IMP]
- regulation of transcription from RNA polymerase II promoter in response to methionine [IMP]
Gene Ontology Molecular Function- RNA polymerase II activating transcription factor binding [IMP, IPI]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- RNA polymerase II repressing transcription factor binding [IDA]
- RNA polymerase II transcription factor binding transcription factor activity [IMP]
- centromeric DNA binding [IDA]
- sequence-specific DNA binding [IDA]
- RNA polymerase II activating transcription factor binding [IMP, IPI]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- RNA polymerase II repressing transcription factor binding [IDA]
- RNA polymerase II transcription factor binding transcription factor activity [IMP]
- centromeric DNA binding [IDA]
- sequence-specific DNA binding [IDA]
Gene Ontology Cellular Component
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Epistatic relationships reveal the functional organization of yeast transcription factors.
The regulation of gene expression is, in large part, mediated by interplay between the general transcription factors (GTFs) that function to bring about the expression of many genes and site-specific DNA-binding transcription factors (STFs). Here, quantitative genetic profiling using the epistatic miniarray profile (E-MAP) approach allowed us to measure 48 391 pairwise genetic interactions, both negative (aggravating) and positive (alleviating), ... [more]
Quantitative Score
- -8.4190433 [SGA Score]
Throughput
- High Throughput
Ontology Terms
- phenotype: colony size (APO:0000063)
Additional Notes
- An Epistatic MiniArray Profile (E-MAP) approach was used to quantitatively score genetic interactions based on fitness defects estimated from the colony size of double versus single mutants. Genetic interactions were considered significant if they had an S score > 2.5 for positive interactions (epistatic or suppressor interactions) and S score < -2.5 for negative interactions (synthetic sick/lethal interactions).
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| CBF1 AFT1 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -6.9335 | BioGRID | 540972 | |
| AFT1 CBF1 | Synthetic Growth Defect Synthetic Growth Defect A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell. | High | - | BioGRID | 442661 | |
| CBF1 AFT1 | Synthetic Growth Defect Synthetic Growth Defect A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell. | Low | - | BioGRID | 195628 | |
| AFT1 CBF1 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | - |
Curated By
- BioGRID