BAIT
PHO2
BAS2, GRF10, phoB, L000000728, YDL106C
Homeobox transcription factor; regulatory targets include genes involved in phosphate metabolism; binds cooperatively with Pho4p to the PHO5 promoter; phosphorylation of Pho2p facilitates interaction with Pho4p; relocalizes to the cytosol in response to hypoxia
GO Process (7)
GO Function (3)
GO Component (2)
Gene Ontology Biological Process
- chromatin remodeling [IMP]
- histidine biosynthetic process [IGI, IMP]
- positive regulation of DNA binding [IDA, IMP]
- positive regulation of phosphate metabolic process [IGI]
- positive regulation of transcription from RNA polymerase II promoter [IGI, IMP]
- positive regulation of transcription regulatory region DNA binding [IMP]
- purine nucleobase biosynthetic process [IDA, IMP]
Gene Ontology Molecular Function
Saccharomyces cerevisiae (S288c)
PREY
UBP3
BLM3, mRNA-binding ubiquitin-specific protease UBP3, L000002417, S000086717, YER151C
Ubiquitin-specific protease involved in transport and osmotic response; interacts with Bre5p to co-regulate anterograde and retrograde transport between the ER and Golgi; involved in transcription elongation in response to osmostress through phosphorylation at Ser695 by Hog1p; inhibitor of gene silencing; cleaves ubiquitin fusions but not polyubiquitin; also has mRNA binding activity; protein abundance increases in response to DNA replication stress; role in ribophagy
GO Process (4)
GO Function (2)
GO Component (1)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Saccharomyces cerevisiae (S288c)
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Epistatic relationships reveal the functional organization of yeast transcription factors.
The regulation of gene expression is, in large part, mediated by interplay between the general transcription factors (GTFs) that function to bring about the expression of many genes and site-specific DNA-binding transcription factors (STFs). Here, quantitative genetic profiling using the epistatic miniarray profile (E-MAP) approach allowed us to measure 48 391 pairwise genetic interactions, both negative (aggravating) and positive (alleviating), ... [more]
Mol. Syst. Biol. Oct. 05, 2010; 6(0);420 [Pubmed: 20959818]
Quantitative Score
- -6.669642162 [SGA Score]
Throughput
- High Throughput
Ontology Terms
- phenotype: colony size (APO:0000063)
Additional Notes
- An Epistatic MiniArray Profile (E-MAP) approach was used to quantitatively score genetic interactions based on fitness defects estimated from the colony size of double versus single mutants. Genetic interactions were considered significant if they had an S score > 2.5 for positive interactions (epistatic or suppressor interactions) and S score < -2.5 for negative interactions (synthetic sick/lethal interactions).
Curated By
- BioGRID