BAIT

SARM1

MyD88-5, SAMD2, SARM

sterile alpha and TIR motif containing 1

Human Papilloma Virus (HPV) Project

GO Process (7)

GO Function (0)

GO Component (6)

Gene Ontology Biological Process

MyD88-independent toll-like receptor signaling pathway [TAS]

innate immune response [TAS]

regulation of dendrite morphogenesis [ISS]

regulation of neuron death [ISS]

toll-like receptor 3 signaling pathway [TAS]

toll-like receptor 4 signaling pathway [TAS]

toll-like receptor signaling pathway [TAS]

Gene Ontology Cellular Component

cytoplasm [ISS]

microtubule [ISS]

mitochondrion [IDA]

CRISPR Database HGNC Alliance of Genome Resources OMIM Entrez Gene RefSeq UniprotKB

Homo sapiens

PREY

TICAM2

MyD88-4, TICAM-2, TIRAP3, TIRP, TRAM

toll-like receptor adaptor molecule 2

GO Process (15)

GO Function (1)

GO Component (5)

Gene Ontology Biological Process

I-kappaB kinase/NF-kappaB signaling [TAS]

MyD88-independent toll-like receptor signaling pathway [TAS]

TRAM-dependent toll-like receptor 4 signaling pathway [IDA, IMP]

TRIF-dependent toll-like receptor signaling pathway [TAS]

cellular response to lipopolysaccharide [IDA, IMP]

innate immune response [TAS]

negative regulation of chemokine (C-C motif) ligand 5 production [IMP]

negative regulation of toll-like receptor 4 signaling pathway [IDA]

positive regulation of I-kappaB kinase/NF-kappaB signaling [IMP]

positive regulation of chemokine (C-C motif) ligand 5 production [IMP]

positive regulation of interleukin-18-mediated signaling pathway [IMP]

positive regulation of toll-like receptor 4 signaling pathway [IDA, IMP]

toll-like receptor 3 signaling pathway [TAS]

toll-like receptor 4 signaling pathway [TAS]

toll-like receptor signaling pathway [TAS]

Gene Ontology Molecular Function

signal transducer activity [IMP]

Gene Ontology Cellular Component

early endosome [IDA]

endoplasmic reticulum [IDA]

endosome membrane [TAS]

late endosome [IDA]

plasma membrane [IDA, TAS]

CRISPR Database HGNC Alliance of Genome Resources OMIM Entrez Gene RefSeq UniprotKB Ensembl

Homo sapiens

PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

MAPPIT analysis of TLR adaptor complexes.

Ulrichts P, Peelman F, Beyaert R, Tavernier J

Toll-like receptors (TLRs) are crucial components of the innate immune system, coupling pathogen recognition to a cellular response. We used the MAPPIT mammalian two-hybrid technique to investigate protein-protein interactions in the early steps in TLR signalling. A partial TLR-adaptor interaction map was constructed confirming several known but also documenting novel interactions. We show that the TLR adaptor Mal is critical ... [more]

FEBS Lett. Feb. 20, 2007; 581(4);629-36 [Pubmed: 17258210]

Throughput

Low Throughput

Additional Notes

interaction detected by MAPPIT

Curated By

BioGRID