RARA
Gene Ontology Biological Process
- apoptotic cell clearance [IMP]
- cellular response to estrogen stimulus [IDA]
- cellular response to retinoic acid [IDA]
- gene expression [TAS]
- intracellular estrogen receptor signaling pathway [IDA]
- negative regulation of granulocyte differentiation [IDA]
- negative regulation of interferon-gamma production [IDA]
- negative regulation of transcription, DNA-templated [IDA]
- negative regulation of tumor necrosis factor production [IDA]
- positive regulation of T-helper 2 cell differentiation [IDA]
- positive regulation of binding [IMP]
- positive regulation of cell cycle [IMP]
- positive regulation of cell proliferation [IMP]
- positive regulation of interleukin-13 production [IDA]
- positive regulation of interleukin-4 production [IDA]
- positive regulation of interleukin-5 production [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IMP]
- positive regulation of transcription, DNA-templated [IDA, IMP]
- protein phosphorylation [IMP]
- response to estradiol [IDA]
- response to retinoic acid [IMP]
- retinoic acid receptor signaling pathway [IMP]
- signal transduction [IDA]
- transcription initiation from RNA polymerase II promoter [TAS]
Gene Ontology Molecular Function- chromatin DNA binding [IDA]
- enzyme binding [IPI]
- protein binding [IPI]
- protein domain specific binding [IPI]
- protein heterodimerization activity [IDA]
- protein kinase A binding [IDA]
- protein kinase B binding [IPI]
- receptor binding [IDA]
- retinoic acid binding [IDA]
- retinoic acid receptor activity [IDA]
- retinoic acid-responsive element binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription coactivator activity [IDA, IMP]
- transcription corepressor activity [IDA]
- transcription factor binding [IPI]
- chromatin DNA binding [IDA]
- enzyme binding [IPI]
- protein binding [IPI]
- protein domain specific binding [IPI]
- protein heterodimerization activity [IDA]
- protein kinase A binding [IDA]
- protein kinase B binding [IPI]
- receptor binding [IDA]
- retinoic acid binding [IDA]
- retinoic acid receptor activity [IDA]
- retinoic acid-responsive element binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription coactivator activity [IDA, IMP]
- transcription corepressor activity [IDA]
- transcription factor binding [IPI]
Gene Ontology Cellular Component
NCOA1
Gene Ontology Biological Process
- androgen receptor signaling pathway [NAS]
- cellular lipid metabolic process [TAS]
- cellular response to hormone stimulus [IBA]
- intracellular receptor signaling pathway [IBA]
- positive regulation of transcription from RNA polymerase II promoter [IDA, NAS]
- positive regulation of transcription from RNA polymerase II promoter by galactose [IDA]
- positive regulation of transcription, DNA-templated [IDA, NAS]
- small molecule metabolic process [TAS]
- transcription, DNA-templated [IDA]
Gene Ontology Molecular Function- RNA polymerase II transcription coactivator activity [NAS]
- androgen receptor binding [NAS]
- enzyme binding [IPI]
- ligand-dependent nuclear receptor binding [IPI]
- ligand-dependent nuclear receptor transcription coactivator activity [IDA]
- nuclear hormone receptor binding [IDA]
- protein N-terminus binding [IPI]
- protein binding [IPI]
- transcription coactivator activity [IDA, NAS]
- RNA polymerase II transcription coactivator activity [NAS]
- androgen receptor binding [NAS]
- enzyme binding [IPI]
- ligand-dependent nuclear receptor binding [IPI]
- ligand-dependent nuclear receptor transcription coactivator activity [IDA]
- nuclear hormone receptor binding [IDA]
- protein N-terminus binding [IPI]
- protein binding [IPI]
- transcription coactivator activity [IDA, NAS]
Gene Ontology Cellular Component
FRET
An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins.
Publication
Ligand-dependent interactions of coactivators steroid receptor coactivator-1 and peroxisome proliferator-activated receptor binding protein with nuclear hormone receptors can be imaged in live cells and are required for transcription.
Members of the nuclear receptor superfamily are thought to activate transcription by recruitment of one or more recently identified coactivator complexes. Here we demonstrate that both peroxisome proliferator-activated receptor binding protein (PBP) and steroid receptor coactivator-1 (SRC-1) are required for ligand-dependent transcription of transiently transfected and chromosomally integrated reporter genes by the estrogen receptor (ER) and retinoic acid receptor (RAR). ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| NCOA1 RARA | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| NCOA1 RARA | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| RARA NCOA1 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | 1417080 | |
| NCOA1 RARA | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| RARA NCOA1 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| RARA NCOA1 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| NCOA1 RARA | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| NCOA1 RARA | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| RARA NCOA1 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| RARA NCOA1 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | 950552 |
Curated By
- BioGRID