An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Gcn1 and actin binding to Yih1: Implications for activation of the eIF2 kinase Gcn2.

Sattlegger E, Barbosa JA, Moraes MC, Martins RM, Hinnebusch AG, Castilho BA

Yeast Yih1 protein and its mammalian ortholog IMPACT, abundant in neurons, are inhibitors of Gcn2, a kinase involved in amino acid homeostasis, stress response, and memory formation. Like Gcn2, Yih1/IMPACT harbors an N-terminal RWD domain that mediates binding to the Gcn2-activator Gcn1. Yih1 competes with Gcn2 for Gcn1-binding, thus inhibiting Gcn2. Yih1 also binds G-actin. Here we show that Yih1-actin ... [more]

Unknown Jan. 14, 2011; 0(0); [Pubmed: 21239490]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
ACT1 GCN1	Positive Genetic Positive Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a less severe fitness defect than expected under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2016)	High	0.1612	BioGRID	1882792

Curated By

BioGRID

Interaction Summary

GCN1

Gene Ontology Biological Process

Gene Ontology Cellular Component

ACT1

Gene Ontology Biological Process

Gene Ontology Molecular Function

histone acetyltransferase activity [IDA]structural constituent of cytoskeleton [IDA]