BAIT

TOP1

MAK1, MAK17, DNA topoisomerase 1, L000002319, YOL006C
Topoisomerase I; nuclear enzyme that relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone; relaxes both positively and negatively supercoiled DNA; functions in replication, transcription, and recombination; role in processing ribonucleoside monophosphates in genomic DNA into irreversible single-strand breaks
Saccharomyces cerevisiae (S288c)
PREY

LTE1

MSI2, mitotic regulator LTE1, L000000955, YAL024C
Protein similar to GDP/GTP exchange factors; without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures
GO Process (3)
GO Function (1)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Publication

Selective ploidy ablation, a high-throughput plasmid transfer protocol, identifies new genes affecting topoisomerase I-induced DNA damage.

Reid RJ, Gonzalez-Barrera S, Sunjevaric I, Alvaro D, Ciccone S, Wagner M, Rothstein R

We have streamlined the process of transferring plasmids into any yeast strain library by developing a novel mating-based, high-throughput method called selective ploidy ablation (SPA). SPA uses a universal plasmid donor strain that contains conditional centromeres on every chromosome. The plasmid-bearing donor is mated to a recipient, followed by removal of all donor-strain chromosomes, producing a haploid strain containing the ... [more]

Genome Res. Mar. 01, 2011; 21(3);477-86 [Pubmed: 21173034]

Throughput

  • High Throughput

Ontology Terms

  • phenotype: vegetative growth (APO:0000106)
  • phenotype: metal resistance (APO:0000090)

Additional Notes

  • Identified by SPA (Synthetic Ploidy Ablation)in the presence of copper induction

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
TOP1 LTE1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.1321BioGRID
413725

Curated By

  • BioGRID