TOP1
Gene Ontology Biological Process
- DNA strand elongation involved in DNA replication [IMP]
- DNA topological change [IDA, IMP]
- chromatin assembly or disassembly [IMP]
- chromatin silencing at rDNA [IMP]
- mitotic chromosome condensation [IGI, IMP]
- nuclear migration [IGI, IMP]
- regulation of mitotic recombination [IMP]
- regulation of transcription from RNA polymerase II promoter [IMP]
- transcription elongation from RNA polymerase II promoter [IMP]
Gene Ontology Molecular Function
PAT1
Gene Ontology Biological Process
- CENP-A containing chromatin organization [IMP]
- chromosome segregation [IMP]
- cytoplasmic mRNA processing body assembly [IGI, IMP]
- deadenylation-dependent decapping of nuclear-transcribed mRNA [IMP, IPI]
- formation of translation preinitiation complex [IMP]
- negative regulation of translational initiation [IDA, IMP]
- regulation of translational initiation [IGI, IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Synthetic Growth Defect
A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.
Publication
Selective ploidy ablation, a high-throughput plasmid transfer protocol, identifies new genes affecting topoisomerase I-induced DNA damage.
We have streamlined the process of transferring plasmids into any yeast strain library by developing a novel mating-based, high-throughput method called selective ploidy ablation (SPA). SPA uses a universal plasmid donor strain that contains conditional centromeres on every chromosome. The plasmid-bearing donor is mated to a recipient, followed by removal of all donor-strain chromosomes, producing a haploid strain containing the ... [more]
Throughput
- High Throughput
Ontology Terms
- phenotype: vegetative growth (APO:0000106)
- phenotype: metal resistance (APO:0000090)
Additional Notes
- Identified by SPA (Synthetic Ploidy Ablation)in the presence of copper induction
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| TOP1 PAT1 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -10.5984 | BioGRID | 214081 | |
| TOP1 PAT1 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.3051 | BioGRID | 413727 | |
| PAT1 TOP1 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.3051 | BioGRID | 361862 | |
| TOP1 PAT1 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.4051 | BioGRID | 2178107 | |
| PAT1 TOP1 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.3517 | BioGRID | 2087825 | |
| TOP1 PAT1 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -12.6226 | BioGRID | 307751 | |
| TOP1 PAT1 | Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition. | High | - | BioGRID | 111737 |
Curated By
- BioGRID