NCOA3
Gene Ontology Biological Process
- androgen receptor signaling pathway [NAS]
- cellular response to hormone stimulus [IBA]
- histone acetylation [IDA]
- intracellular receptor signaling pathway [IBA]
- positive regulation of gene expression [IMP]
- positive regulation of keratinocyte differentiation [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IMP]
- positive regulation of transcription, DNA-templated [NAS]
- receptor transactivation [TAS]
- regulation of RNA biosynthetic process [IMP]
Gene Ontology Molecular Function- androgen receptor binding [NAS]
- histone acetyltransferase activity [IDA]
- ligand-dependent nuclear receptor binding [IPI]
- ligand-dependent nuclear receptor transcription coactivator activity [IBA]
- nuclear hormone receptor binding [IDA]
- protein N-terminus binding [IPI]
- protein binding [IPI]
- thyroid hormone receptor binding [NAS]
- transcription coactivator activity [IDA, IMP, NAS]
- androgen receptor binding [NAS]
- histone acetyltransferase activity [IDA]
- ligand-dependent nuclear receptor binding [IPI]
- ligand-dependent nuclear receptor transcription coactivator activity [IBA]
- nuclear hormone receptor binding [IDA]
- protein N-terminus binding [IPI]
- protein binding [IPI]
- thyroid hormone receptor binding [NAS]
- transcription coactivator activity [IDA, IMP, NAS]
Gene Ontology Cellular Component
VDR
Gene Ontology Biological Process
- bile acid signaling pathway [IDA]
- cell morphogenesis [IMP]
- decidualization [IEP]
- gene expression [TAS]
- negative regulation of cell proliferation [IDA]
- negative regulation of keratinocyte proliferation [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IDA]
- negative regulation of transcription, DNA-templated [IDA]
- positive regulation of gene expression [IMP]
- positive regulation of keratinocyte differentiation [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IMP]
- positive regulation of vitamin D 24-hydroxylase activity [IDA]
- regulation of calcidiol 1-monooxygenase activity [ISS]
- signal transduction [TAS]
- transcription initiation from RNA polymerase II promoter [TAS]
- vitamin D receptor signaling pathway [IDA]
Gene Ontology Molecular Function- DNA binding [IDA]
- calcitriol binding [IDA]
- calcitriol receptor activity [IDA]
- lithocholic acid binding [IDA]
- lithocholic acid receptor activity [IDA]
- protein binding [IPI]
- retinoid X receptor binding [IPI]
- sequence-specific DNA binding transcription factor activity [IDA]
- vitamin D response element binding [IDA]
- DNA binding [IDA]
- calcitriol binding [IDA]
- calcitriol receptor activity [IDA]
- lithocholic acid binding [IDA]
- lithocholic acid receptor activity [IDA]
- protein binding [IPI]
- retinoid X receptor binding [IPI]
- sequence-specific DNA binding transcription factor activity [IDA]
- vitamin D response element binding [IDA]
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Lysine 246 of the vitamin D receptor is crucial for ligand-dependent interaction with coactivators and transcriptional activity.
Mutant K246A in the predicted helix 3 of the ligand-binding domain, as well as mutants L417S and E420Q in helix 12, which contains the core ligand-dependent transcriptional activation domain (AF-2), were generated to examine AF-2 activity of the vitamin D receptor (VDR). These mutations abolished vitamin D-dependent transactivation. In addition, VDR mediates a ligand-dependent repression of the response of the ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| VDR NCOA3 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| VDR NCOA3 | Co-localization Co-localization Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments. | Low | - | BioGRID | 503232 | |
| NCOA3 VDR | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - | |
| VDR NCOA3 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | 1056003 | |
| VDR NCOA3 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID