BAIT
MAPK1
9030612K14Rik, AA407128, AU018647, C78273, ERK, Erk2, MAPK2, PRKM2, Prkm1, p41mapk, p42mapk
mitogen-activated protein kinase 1
GO Process (35)
GO Function (12)
GO Component (18)
Gene Ontology Biological Process
- B cell receptor signaling pathway [IDA]
- ERBB signaling pathway [ISO]
- ERK1 and ERK2 cascade [ISO]
- MAPK cascade [IDA, IMP, ISO]
- MAPK import into nucleus [ISO]
- T cell receptor signaling pathway [IDA]
- caveolin-mediated endocytosis [TAS]
- cellular response to DNA damage stimulus [IDA]
- cellular response to granulocyte macrophage colony-stimulating factor stimulus [IDA]
- cellular response to organic substance [ISO]
- cytosine metabolic process [IDA]
- intracellular signal transduction [ISO]
- labyrinthine layer blood vessel development [IMP]
- lipopolysaccharide-mediated signaling pathway [IDA]
- mammary gland epithelial cell proliferation [IDA]
- negative regulation of cell differentiation [IGI]
- organ morphogenesis [IDA]
- peptidyl-serine phosphorylation [IDA, IMP, ISO]
- peptidyl-threonine phosphorylation [IDA]
- positive regulation of peptidyl-threonine phosphorylation [ISO]
- positive regulation of translation [ISO]
- protein phosphorylation [IDA, IMP, ISO]
- regulation of Golgi inheritance [TAS]
- regulation of cytoskeleton organization [TAS]
- regulation of early endosome to late endosome transport [TAS]
- regulation of sequence-specific DNA binding transcription factor activity [NAS]
- regulation of stress-activated MAPK cascade [TAS]
- response to epidermal growth factor [ISO]
- response to estrogen [ISO]
- response to exogenous dsRNA [IDA]
- response to lipopolysaccharide [IDA]
- response to toxic substance [ISO]
- sensory perception of pain [ISO]
- signal transduction [ISO]
- transcription, DNA-templated [NAS]
Gene Ontology Molecular Function- ATP binding [ISO]
- MAP kinase activity [IDA, IMP, ISO]
- RNA polymerase II carboxy-terminal domain kinase activity [IDA]
- kinase activity [IDA]
- mitogen-activated protein kinase kinase kinase binding [ISO]
- phosphatase binding [ISO]
- phosphotyrosine binding [IMP]
- protein binding [IPI]
- protein kinase activity [IDA]
- protein kinase binding [ISO]
- protein serine/threonine kinase activity [IDA, ISO]
- transcription factor binding [ISO]
- ATP binding [ISO]
- MAP kinase activity [IDA, IMP, ISO]
- RNA polymerase II carboxy-terminal domain kinase activity [IDA]
- kinase activity [IDA]
- mitogen-activated protein kinase kinase kinase binding [ISO]
- phosphatase binding [ISO]
- phosphotyrosine binding [IMP]
- protein binding [IPI]
- protein kinase activity [IDA]
- protein kinase binding [ISO]
- protein serine/threonine kinase activity [IDA, ISO]
- transcription factor binding [ISO]
Gene Ontology Cellular Component
- Golgi apparatus [TAS]
- axon [ISO]
- caveola [TAS]
- cytoplasm [IDA, ISO]
- cytoskeleton [TAS]
- cytosol [IDA, ISO, TAS]
- dendrite cytoplasm [ISO]
- early endosome [TAS]
- extracellular vesicular exosome [ISO]
- focal adhesion [TAS]
- late endosome [TAS]
- microtubule cytoskeleton [ISO]
- mitochondrion [IDA, TAS]
- nucleoplasm [ISO]
- nucleus [IDA, ISO, TAS]
- perikaryon [ISO]
- protein complex [ISO]
- pseudopodium [IDA]
Mus musculus
PREY
TOP2A
Top-2, RP23-333D2.5
topoisomerase (DNA) II alpha
GO Process (18)
GO Function (19)
GO Component (8)
Gene Ontology Biological Process
- ATP catabolic process [IGI, ISO]
- DNA ligation [ISO]
- DNA topological change [IDA, ISO]
- DNA unwinding involved in DNA replication [IBA]
- apoptotic chromosome condensation [IBA, ISO]
- cellular response to DNA damage stimulus [ISO]
- chromosome condensation [IMP]
- chromosome segregation [IDA, IMP, ISO]
- embryonic cleavage [IMP]
- hematopoietic progenitor cell differentiation [IMP]
- mitotic DNA integrity checkpoint [IBA]
- mitotic recombination [IBA]
- positive regulation of apoptotic process [ISO]
- positive regulation of single stranded viral RNA replication via double stranded DNA intermediate [ISO]
- positive regulation of transcription from RNA polymerase II promoter [IMP]
- positive regulation of viral genome replication [ISO]
- resolution of meiotic recombination intermediates [IBA]
- sister chromatid segregation [IBA]
Gene Ontology Molecular Function- ATP binding [ISO]
- DNA binding [ISO]
- DNA binding, bending [ISO]
- DNA topoisomerase activity [ISO]
- DNA topoisomerase type II (ATP-hydrolyzing) activity [IDA, IGI, ISO]
- DNA-dependent ATPase activity [ISO]
- chromatin binding [ISO]
- drug binding [ISO]
- enzyme binding [ISO]
- histone deacetylase binding [ISO]
- magnesium ion binding [ISO]
- poly(A) RNA binding [ISO]
- protein C-terminus binding [ISO]
- protein heterodimerization activity [ISO]
- protein homodimerization activity [ISO]
- protein kinase C binding [ISO]
- sequence-specific DNA binding [ISO]
- structure-specific DNA binding [ISO]
- ubiquitin binding [ISO]
- ATP binding [ISO]
- DNA binding [ISO]
- DNA binding, bending [ISO]
- DNA topoisomerase activity [ISO]
- DNA topoisomerase type II (ATP-hydrolyzing) activity [IDA, IGI, ISO]
- DNA-dependent ATPase activity [ISO]
- chromatin binding [ISO]
- drug binding [ISO]
- enzyme binding [ISO]
- histone deacetylase binding [ISO]
- magnesium ion binding [ISO]
- poly(A) RNA binding [ISO]
- protein C-terminus binding [ISO]
- protein heterodimerization activity [ISO]
- protein homodimerization activity [ISO]
- protein kinase C binding [ISO]
- sequence-specific DNA binding [ISO]
- structure-specific DNA binding [ISO]
- ubiquitin binding [ISO]
Gene Ontology Cellular Component
Mus musculus
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Extracellular signal-regulated kinase activates topoisomerase IIalpha through a mechanism independent of phosphorylation.
The mitogen-activated protein (MAP) kinases, extracellular signal-related kinase 1 (ERK1) and ERK2, regulate cellular responses by mediating extracellular growth signals toward cytoplasmic and nuclear targets. A potential target for ERK is topoisomerase IIalpha, which becomes highly phosphorylated during mitosis and is required for several aspects of nucleic acid metabolism, including chromosome condensation and daughter chromosome separation. In this study, we ... [more]
Mol. Cell. Biol. May. 01, 1999; 19(5);3551-60 [Pubmed: 10207078]
Throughput
- Low Throughput
Curated By
- BioGRID