BAIT

HMGA1

HMG-R, HMGA1A, HMGIY, RP11-513I15.2
high mobility group AT-hook 1
Homo sapiens

Far Western

An interaction is detected between a protein immobilized on a membrane and a purified protein probe.

Publication

High mobility group I/Y protein functions as a specific cofactor for Oct-2A: mapping of interaction domains.

Abdulkadir SA, Casolaro V, Tai AK, Thanos D, Ono SJ

The octamer motif (ATTTGCAT) present in several eukaryotic promoters and enhancers is now known to influence the transcription of several genes by interacting with members of a broad family of homeodomain proteins. The promoter of the human class II MHC gene HLA-DRA contains a conserved octamer element that can bind (among other proteins) the transcription factor Oct-2A and the high ... [more]

J. Leukoc. Biol. Nov. 01, 1998; 64(5);681-91 [Pubmed: 9823775]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
HMGA1 POU2F1
Reconstituted Complex
Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Low-BioGRID
-

Curated By

  • BioGRID