BAIT
CRZ1
HAL8, TCN1, DNA-binding transcription factor CRZ1, L000004202, YNL027W
Transcription factor, activates transcription of stress response genes; nuclear localization is positively regulated by calcineurin-mediated dephosphorylation; rapidly localizes to the nucleus under blue light stress; can be activated in stochastic pulses of nuclear localization in response to calcium
GO Process (12)
GO Function (3)
GO Component (2)
Gene Ontology Biological Process
- cell wall chitin biosynthetic process [IGI]
- cellular response to blue light [IDA]
- ion homeostasis [IGI, IMP]
- positive regulation of cellular response to drug [IMP]
- positive regulation of sodium ion transport by positive regulation of transcription from RNA polymerase II promoter [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IGI, IMP]
- positive regulation of transcription from RNA polymerase II promoter by calcium-mediated signaling [IGI, IMP]
- positive regulation of transcription from RNA polymerase II promoter by pheromones [IMP]
- positive regulation of transcription from RNA polymerase II promoter in response to alkaline pH [IMP]
- positive regulation of transcription from RNA polymerase II promoter in response to calcium ion [IMP]
- positive regulation of transcription from RNA polymerase II promoter in response to increased salt [IMP]
- regulation of potassium ion concentration by positive regulation of transcription from RNA polymerase II promoter [IDA, IGI, IMP]
Gene Ontology Molecular Function
Saccharomyces cerevisiae (S288c)
PREY
HKR1
L000000788, YDR420W
Mucin family member that functions as an osmosensor in the HOG pathway; functions in the Sho1p-mediated HOG pathway with Msb2p; proposed to be a negative regulator of filamentous growth; mutant displays defects in beta-1,3 glucan synthesis and bud site selection
GO Process (5)
GO Function (1)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
A plasma-membrane E-MAP reveals links of the eisosome with sphingolipid metabolism and endosomal trafficking.
The plasma membrane delimits the cell and controls material and information exchange between itself and the environment. How different plasma-membrane processes are coordinated and how the relative abundance of plasma-membrane lipids and proteins is homeostatically maintained are not yet understood. Here, we used a quantitative genetic interaction map, or E-MAP, to functionally interrogate a set of approximately 400 genes involved ... [more]
Nat. Struct. Mol. Biol. Jul. 01, 2010; 17(7);901-8 [Pubmed: 20526336]
Quantitative Score
- -10.974882 [SGA Score]
Throughput
- High Throughput
Ontology Terms
- phenotype: colony size (APO:0000063)
Additional Notes
- An Epistatic MiniArray Profile (E-MAP) approach was used to quantitatively score genetic interactions based on fitness defects estimated from the colony size of double versus single mutants. Genetic interactions were considered significant if they had an S score > 2.5 for positive interactions (epistatic or suppressor interactions) and S score < -2.5 for negative interactions (synthetic sick/lethal interactions).
Curated By
- BioGRID