TRIM28
Gene Ontology Biological Process
- DNA methylation involved in embryo development [IMP]
- DNA repair [ISO]
- convergent extension involved in axis elongation [IMP]
- embryo implantation [IMP]
- embryonic placenta morphogenesis [IMP]
- epithelial to mesenchymal transition [IDA]
- in utero embryonic development [IMP]
- innate immune response [ISO]
- negative regulation of DNA demethylation [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IDA, IGI]
- negative regulation of transcription, DNA-templated [IDA, IMP, ISO]
- negative regulation of viral release from host cell [ISO]
- positive regulation of DNA repair [ISO]
- positive regulation of transcription factor import into nucleus [ISO]
- positive regulation of transcription, DNA-templated [IDA]
- protein autophosphorylation [IDA]
- protein oligomerization [ISO]
- protein phosphorylation [IDA]
- protein sumoylation [ISO]
- protein ubiquitination [ISO]
- regulation of genetic imprinting [IMP]
Gene Ontology Molecular Function- DNA binding [IDA, ISO]
- Krueppel-associated box domain binding [ISO]
- chromo shadow domain binding [ISO]
- poly(A) RNA binding [ISO]
- protein binding [IPI]
- protein kinase activity [IDA]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription coactivator activity [IDA]
- transcription corepressor activity [ISO]
- ubiquitin protein ligase binding [ISO]
- ubiquitin-protein transferase activity [ISO]
- zinc ion binding [ISO]
- DNA binding [IDA, ISO]
- Krueppel-associated box domain binding [ISO]
- chromo shadow domain binding [ISO]
- poly(A) RNA binding [ISO]
- protein binding [IPI]
- protein kinase activity [IDA]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription coactivator activity [IDA]
- transcription corepressor activity [ISO]
- ubiquitin protein ligase binding [ISO]
- ubiquitin-protein transferase activity [ISO]
- zinc ion binding [ISO]
Gene Ontology Cellular Component
MYB
Gene Ontology Biological Process
- B cell differentiation [IMP]
- G1/S transition of mitotic cell cycle [IMP]
- calcium ion transport [IMP]
- cellular response to interleukin-6 [IDA]
- chromatin remodeling [ISO]
- embryonic digestive tract development [IMP]
- homeostasis of number of cells [IMP]
- in utero embryonic development [IMP]
- negative regulation of transcription from RNA polymerase II promoter [ISO]
- negative regulation of transcription, DNA-templated [ISO]
- positive regulation of T-helper cell differentiation [ISO]
- positive regulation of histone H3-K4 methylation [ISO]
- positive regulation of histone H3-K9 methylation [ISO]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IGI, ISO]
- positive regulation of transcription, DNA-templated [ISO]
- regulation of gene expression [IMP]
- regulation of transcription, DNA-templated [IDA, IMP]
- thymus development [IMP]
- transcription from RNA polymerase II promoter [IDA]
Gene Ontology Molecular Function- DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA, ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- WD40-repeat domain binding [IDA]
- protein binding [IPI]
- sequence-specific DNA binding transcription factor activity [IDA, IMP]
- DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA, ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- WD40-repeat domain binding [IDA]
- protein binding [IPI]
- sequence-specific DNA binding transcription factor activity [IDA, IMP]
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Oncogenic activation of c-Myb correlates with a loss of negative regulation by TIF1beta and Ski.
The c-myb proto-oncogene product (c-Myb) regulates proliferation of hematopoietic cells by inducing the transcription of a group of target genes. Removal or mutations of the negative regulatory domain (NRD) in the C-terminal half of c-Myb leads to increased transactivating capacity and oncogenic activation. Here we report that TIF1beta directly binds to the NRD and negatively regulates the c-Myb-dependent trans-activation. In ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| MYB TRIM28 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID