BAIT

BAZ1A

ACF1, WALp1, WCRF180, hACF1, HSPC317
bromodomain adjacent to zinc finger domain, 1A
GO Process (4)
GO Function (2)
GO Component (2)
Homo sapiens
PREY

POLE3

CHARAC17, CHRAC17, YBL1, p17
polymerase (DNA directed), epsilon 3, accessory subunit
GO Process (3)
GO Function (1)
GO Component (3)
Homo sapiens

Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Publication

The histone-fold protein complex CHRAC-15/17 enhances nucleosome sliding and assembly mediated by ACF.

Kukimoto I, Elderkin S, Grimaldi M, Oelgeschlaeger T, Varga-Weisz PD

The histone fold is a structural motif with which two related proteins interact and is found in complexes involved in wrapping DNA, the nucleosome, and transcriptional regulation, as in NC2. We reveal a novel function for histone-fold proteins: facilitation of nucleosome remodeling. ACF1-ISWI complex (ATP-dependent chromatin assembly and remodeling factor [ACF]) associates with histone-fold proteins (CHRAC-15 and CHRAC-17 in the ... [more]

Mol. Cell Jan. 30, 2004; 13(2);265-77 [Pubmed: 14759371]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
POLE3 BAZ1A
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High1BioGRID
2274387
POLE3 BAZ1A
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-
BAZ1A POLE3
Positive Genetic
Positive Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a less severe fitness defect than expected under a given condition. This term is reserved for high or low throughput studies with scores.

High3.412BioGRID
2539568
POLE3 BAZ1A
Reconstituted Complex
Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Low-BioGRID
-

Curated By

  • BioGRID