CDKN1A
Gene Ontology Biological Process
- DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest [ISO]
- G1/S transition of mitotic cell cycle [ISO]
- G2/M transition of mitotic cell cycle [ISO]
- cell cycle arrest [IDA, ISO]
- cellular response to DNA damage stimulus [IMP, ISO]
- cellular response to extracellular stimulus [ISO]
- cellular response to ionizing radiation [ISO]
- cellular senescence [ISO]
- intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator [IMP]
- mitotic cell cycle arrest [IDA]
- negative regulation of G1/S transition of mitotic cell cycle [ISO]
- negative regulation of apoptotic process [ISO]
- negative regulation of cell growth [ISO]
- negative regulation of cell proliferation [IMP, ISO]
- negative regulation of cyclin-dependent protein serine/threonine kinase activity [IDA]
- negative regulation of gene expression [IMP]
- negative regulation of phosphorylation [ISO]
- positive regulation of B cell proliferation [IGI]
- positive regulation of fibroblast proliferation [ISO]
- positive regulation of programmed cell death [IMP]
- positive regulation of protein kinase activity [ISO]
- positive regulation of reactive oxygen species metabolic process [ISO]
- protein phosphorylation [ISO]
- regulation of DNA biosynthetic process [IMP]
- regulation of cell cycle [IDA]
- regulation of cyclin-dependent protein serine/threonine kinase activity [IMP]
- regulation of mitotic cell cycle [IMP]
- regulation of protein import into nucleus, translocation [IMP]
- response to UV [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
CDK1
Gene Ontology Biological Process
- cell aging [ISO]
- chromosome condensation [ISO]
- histone phosphorylation [ISO]
- mitotic G2 DNA damage checkpoint [IDA]
- negative regulation of apoptotic process [ISO]
- organ regeneration [ISO]
- peptidyl-serine phosphorylation [ISO]
- peptidyl-threonine phosphorylation [ISO]
- positive regulation of DNA replication [ISO]
- positive regulation of cardiac muscle cell proliferation [ISO]
- positive regulation of gene expression [ISO]
- positive regulation of mitotic cell cycle [ISO]
- positive regulation of protein import into nucleus, translocation [ISO]
- protein complex assembly [ISO]
- protein localization to kinetochore [ISO]
- protein phosphorylation [IDA]
- response to ethanol [ISO]
- response to organonitrogen compound [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Mutant MyoD lacking Cdc2 phosphorylation sites delays M-phase entry.
The transcription factors MyoD and Myf-5 control myoblast identity and differentiation. MyoD and Myf-5 manifest opposite cell cycle-specific expression patterns. Here, we provide evidence that MyoD plays a pivotal role at the G(2)/M transition by controlling the expression of p21(Waf1/Cip1) (p21), which is believed to regulate cyclin B-Cdc2 kinase activity in G(2). In growing myoblasts, MyoD reaccumulates during G(2) concomitantly ... [more]
Throughput
- Low Throughput
Additional Notes
- CCNB
Curated By
- BioGRID