BAIT

CDK2

eg1
cyclin-dependent kinase 2
GO Process (0)
GO Function (0)
GO Component (0)
Xenopus laevis
PREY

CDKNX

Xic-1, Xic1, p27XIC1, p28
cyclin-dependent kinase inhibitor xic1
GO Process (0)
GO Function (0)
GO Component (0)
Xenopus laevis

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Xic1 degradation in Xenopus egg extracts is coupled to initiation of DNA replication.

You Z, Harvey K, Kong L, Newport J

CDK2 activity is regulated by phosphorylation/dephosphorylation, subcellular localization, cyclin levels, and cyclin dependent kinase inhibitors (CKIs). Using Xenopus egg extracts, we find that degradation of Xic1, a Xenopus p21(cip1)/p27(kip1) family member, is coupled to initiation of DNA replication. Xic1 turnover requires the formation of a prereplication complex (pre-RC). Additionally, downstream initiation factors including CDK2, Cdc7, and Cdc45, but not RPA ... [more]

Genes Dev. May. 15, 2002; 16(10);1182-94 [Pubmed: 12023298]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
CDKNX CDK2
Reconstituted Complex
Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Low-BioGRID
-

Curated By

  • BioGRID