BAIT

ENT1

YDL161W
Epsin-like protein involved in endocytosis and actin patch assembly; functionally redundant with Ent2p; binds clathrin via a clathrin-binding domain motif at C-terminus; relocalizes from bud neck to cytoplasm upon DNA replication stress; ENT1 has a paralog, ENT2, that arose from the whole genome duplication
GO Process (3)
GO Function (1)
GO Component (5)
Saccharomyces cerevisiae (S288c)
PREY

EDE1

BUD15, YBL047C
Scaffold protein involved in the formation of early endocytic sites; putative regulator of cytokinesis; homo-oligomerization is required for localization to and organization of endocytic sites; has a network of interactions with other endocytic proteins; binds membranes in a ubiquitin-dependent manner; may also bind ubiquitinated membrane-associated proteins; interacts with Cmk2 and functions upstream of CMK2 in regulating non-apoptotic cell death; homolog of mammalian Eps15
GO Process (4)
GO Function (1)
GO Component (5)
Saccharomyces cerevisiae (S288c)

Phenotypic Enhancement

A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

Epsins and Vps27p/Hrs contain ubiquitin-binding domains that function in receptor endocytosis.

Shih SC, Katzmann DJ, Schnell JD, Sutanto M, Emr SD, Hicke L

Ubiquitin functions as a signal for sorting cargo at multiple steps of the endocytic pathway and controls the activity of trans-acting components of the endocytic machinery (reviewed in refs 1, and 2). By contrast to proteasome degradation, which generally requires a polyubiquitin chain that is at least four subunits long, internalization and sorting of endocytic cargo at the late endosome ... [more]

Nat. Cell Biol. May. 01, 2002; 4(5);389-93 [Pubmed: 11988742]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: protein/peptide distribution (APO:0000209)

Additional Notes

  • double mutants show synthetic defects in alpha-factor internalization

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
ENT1 EDE1
Reconstituted Complex
Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Low-BioGRID
-
ENT1 EDE1
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID