BAIT
URN1
YPR152C
Putative protein of unknown function containing WW and FF domains; overexpression causes accumulation of cells in G1 phase
Saccharomyces cerevisiae (S288c)
PREY
FAS1
tetrafunctional fatty acid synthase subunit FAS1, L000000601, YKL182W
Beta subunit of fatty acid synthetase; complex catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities
GO Process (1)
GO Function (6)
GO Component (5)
Gene Ontology Biological Process
Gene Ontology Molecular Function- 3-hydroxyacyl-[acyl-carrier-protein] dehydratase activity [IDA, IMP]
- [acyl-carrier-protein] S-acetyltransferase activity [IDA, IMP]
- [acyl-carrier-protein] S-malonyltransferase activity [IDA, IMP]
- enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) activity [IDA, IMP]
- fatty acid synthase activity [IMP]
- palmitoyltransferase activity [IDA, IMP]
- 3-hydroxyacyl-[acyl-carrier-protein] dehydratase activity [IDA, IMP]
- [acyl-carrier-protein] S-acetyltransferase activity [IDA, IMP]
- [acyl-carrier-protein] S-malonyltransferase activity [IDA, IMP]
- enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) activity [IDA, IMP]
- fatty acid synthase activity [IMP]
- palmitoyltransferase activity [IDA, IMP]
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Systematic Two-Hybrid and Comparative Proteomic Analyses Reveal Novel Yeast Pre-mRNA Splicing Factors Connected to Prp19.
Prp19 is the founding member of the NineTeen Complex, or NTC, which is a spliceosomal subcomplex essential for spliceosome activation. To define Prp19 connectivity and dynamic protein interactions within the spliceosome, we systematically queried the Saccharomyces cerevisiae proteome for Prp19 WD40 domain interaction partners by two-hybrid analysis. We report that in addition to S. cerevisiae Cwc2, the splicing factor Prp17 ... [more]
PLoS ONE Mar. 10, 2011; 6(2);e16719 [Pubmed: 21386897]
Throughput
- High Throughput
Curated By
- BioGRID