OSH3
Gene Ontology Biological Process
- ER to Golgi ceramide transport [IMP]
- endocytosis [IGI]
- exocytosis [IGI]
- invasive growth in response to glucose limitation [IGI]
- karyogamy involved in conjugation with cellular fusion [IGI]
- maintenance of cell polarity [IGI]
- positive regulation of phosphatase activity [IDA]
- pseudohyphal growth [IMP]
- sterol transport [IDA, IGI, IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
SAC1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Biochemical Activity
An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.
Publication
Osh proteins regulate phosphoinositide metabolism at ER-plasma membrane contact sites.
Sac1 phosphoinositide (PI) phosphatases are essential regulators of PI-signaling networks. Yeast Sac1, an integral endoplasmic reticulum (ER) membrane protein, controls PI4P levels at the ER, Golgi, and plasma membrane (PM). Whether Sac1 can act in trans and turn over PI4P at the Golgi and PM from the ER remains a paradox. We find that Sac1-mediated PI4P metabolism requires the oxysterol-binding ... [more]
Throughput
- Low Throughput
Additional Notes
- Osh3 or Osh4 stimulate Sac1 phosphatase activity in vitro.
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
OSH3 SAC1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | 518727 |
Curated By
- BioGRID