BAIT
THP1
BUD29, YOL072W
Nuclear pore-associated protein; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; contains a PAM domain implicated in protein-protein binding
GO Process (7)
GO Function (2)
GO Component (4)
Gene Ontology Biological Process
- cellular bud site selection [IMP]
- mRNA 3'-end processing [IMP]
- mRNA export from nucleus [IMP]
- nuclear retention of pre-mRNA at the site of transcription [IMP]
- posttranscriptional tethering of RNA polymerase II gene DNA at nuclear periphery [IMP]
- transcription elongation from RNA polymerase II promoter [IMP]
- transcription-coupled nucleotide-excision repair [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
PGD1
HRS1, MED3, L000000811, L000001408, YGL025C
Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor
GO Process (3)
GO Function (3)
GO Component (1)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
Phenotypic Suppression
A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.
Publication
A reduction in RNA polymerase II initiation rate suppresses hyper-recombination and transcription-elongation impairment of THO mutants.
Hrs1/Med3, a component of the Mediator involved in transcription initiation, was previously isolated as a suppressor of hpr1Delta hyper-recombination linked to transcription elongation. Here we show that hrs1Delta-mediated suppression is specific of transcription-associated hyper-recombination (TAR). The decrease in recombination associated with hrs1Delta, either in wild-type or hpr1Delta cells is only observed in DNA repeats constructs in which transcription is Hrs1-dependent. ... [more]
Mol. Genet. Genomics Oct. 01, 2008; 280(4);327-36 [Pubmed: 18682986]
Throughput
- Low Throughput
Ontology Terms
- phenotype: mitotic recombination (APO:0000225)
Additional Notes
- deletion of med3 suppresses the transcription -associated hyper-recombination seen in a hpr1, tho2 or thp1 mutant
Related interactions
Curated By
- BioGRID