BAIT
MDM2
ACTFS, HDMX, hdm2
MDM2 proto-oncogene, E3 ubiquitin protein ligase
GO Process (25)
GO Function (7)
GO Component (9)
Gene Ontology Biological Process
- DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest [IMP, TAS]
- Fc-epsilon receptor signaling pathway [TAS]
- cellular response to hypoxia [IEP]
- epidermal growth factor receptor signaling pathway [TAS]
- establishment of protein localization [IDA]
- fibroblast growth factor receptor signaling pathway [TAS]
- innate immune response [TAS]
- negative regulation of DNA damage response, signal transduction by p53 class mediator [IDA]
- negative regulation of cell cycle arrest [IDA]
- negative regulation of transcription from RNA polymerase II promoter [IDA]
- negative regulation of transcription, DNA-templated [IDA]
- neurotrophin TRK receptor signaling pathway [TAS]
- peptidyl-lysine modification [IMP]
- phosphatidylinositol-mediated signaling [TAS]
- positive regulation of cell proliferation [TAS]
- positive regulation of mitotic cell cycle [IMP]
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process [IDA]
- protein complex assembly [IDA]
- protein destabilization [IDA]
- protein localization to nucleus [IDA]
- protein ubiquitination [IDA]
- protein ubiquitination involved in ubiquitin-dependent protein catabolic process [IDA]
- regulation of protein catabolic process [IDA]
- response to antibiotic [IEP]
- synaptic transmission [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
CDKN2A
ARF-INK4a, Arf, INK4a-ARF, Ink4a/Arf, MTS1, Pctr1, p16, p16(INK4a), p16INK4a, p19, p19ARF, RP23-166I8.5
cyclin-dependent kinase inhibitor 2A
GO Process (57)
GO Function (10)
GO Component (8)
Gene Ontology Biological Process
- G1/S transition of mitotic cell cycle [ISO]
- activation of cysteine-type endopeptidase activity involved in apoptotic process [ISO]
- aging [IMP]
- apoptotic mitochondrial changes [ISO]
- cell aging [IDA]
- cell cycle arrest [IDA, ISO]
- cellular response to hydrogen peroxide [IDA]
- cellular senescence [IDA, ISO]
- epidermis development [IMP]
- glucose homeostasis [IMP]
- negative regulation of B cell proliferation [IMP]
- negative regulation of NF-kappaB transcription factor activity [ISO]
- negative regulation of cell cycle [IDA]
- negative regulation of cell growth [IMP, ISO]
- negative regulation of cell proliferation [ISO]
- negative regulation of cell-matrix adhesion [ISO]
- negative regulation of cyclin-dependent protein serine/threonine kinase activity [IDA, ISO]
- negative regulation of immature T cell proliferation in thymus [IMP]
- negative regulation of mammary gland epithelial cell proliferation [IMP]
- negative regulation of phosphorylation [ISO]
- negative regulation of protein binding [IDA]
- negative regulation of protein kinase activity [ISO]
- negative regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process [IDA]
- negative regulation of proteolysis involved in cellular protein catabolic process [IDA]
- negative regulation of transcription, DNA-templated [ISO]
- negative regulation of ubiquitin-protein transferase activity [IDA]
- positive regulation of DNA damage response, signal transduction by p53 class mediator [IC, ISO]
- positive regulation of apoptotic process [ISO]
- positive regulation of apoptotic process involved in mammary gland involution [IMP]
- positive regulation of cell cycle arrest [ISO]
- positive regulation of cellular senescence [ISO]
- positive regulation of macrophage apoptotic process [IGI]
- positive regulation of protein sumoylation [ISO]
- positive regulation of sequence-specific DNA binding transcription factor activity [IMP]
- positive regulation of smooth muscle cell apoptotic process [IGI]
- positive regulation of transcription from RNA polymerase II promoter [ISO]
- positive regulation of transcription, DNA-templated [IMP, ISO]
- protein K63-linked ubiquitination [ISO]
- protein destabilization [ISO]
- protein polyubiquitination [ISO]
- protein stabilization [ISO]
- rRNA transcription [IGI]
- regulation of G2/M transition of mitotic cell cycle [ISO]
- regulation of apoptotic DNA fragmentation [ISO]
- regulation of cyclin-dependent protein serine/threonine kinase activity [IDA]
- regulation of gene expression [IMP]
- regulation of nucleocytoplasmic transport [IGI]
- regulation of protein export from nucleus [ISO]
- regulation of protein stability [IMP]
- regulation of transcription, DNA-templated [IDA]
- replicative senescence [ISO]
- response to drug [ISO]
- response to organic cyclic compound [ISO]
- response to organonitrogen compound [ISO]
- senescence-associated heterochromatin focus assembly [ISO]
- somatic stem cell division [IMP]
- somatic stem cell maintenance [IGI]
Gene Ontology Molecular Function- MDM2/MDM4 family protein binding [ISO]
- NF-kappaB binding [ISO]
- cyclin-dependent protein serine/threonine kinase inhibitor activity [IDA, ISO]
- p53 binding [ISO]
- poly(A) RNA binding [ISO]
- protein binding [IPI]
- protein kinase binding [ISO]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription factor binding [ISO]
- ubiquitin-protein transferase inhibitor activity [IDA]
- MDM2/MDM4 family protein binding [ISO]
- NF-kappaB binding [ISO]
- cyclin-dependent protein serine/threonine kinase inhibitor activity [IDA, ISO]
- p53 binding [ISO]
- poly(A) RNA binding [ISO]
- protein binding [IPI]
- protein kinase binding [ISO]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription factor binding [ISO]
- ubiquitin-protein transferase inhibitor activity [IDA]
Gene Ontology Cellular Component
Mus musculus
Reconstituted Complex
An interaction is detected between purified proteins in vitro.
Publication
Defining the molecular basis of Arf and Hdm2 interactions.
Understanding the interaction of Arf and Hdm2 has recently become a central issue in cancer biology. In response to hyperproliferative signals, p14(Arf) stabilizes p53 by binding to Hdm2 and inhibits the ubiquitination and subsequent proteosome-dependent degradation of p53. The medical importance of the Arf-Hdm2-p53 regulatory system is highlighted by the finding that either p53 or p14(Arf) are lost or modified ... [more]
J. Mol. Biol. Nov. 23, 2001; 314(2);263-77 [Pubmed: 11718560]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| MDM2 CDKN2A | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID