Biochemical Activity (Phosphorylation)

An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.

Publication

Casein kinase II-mediated phosphorylation of general repressor Maf1 triggers RNA polymerase III activation.

Graczyk D, Debski J, Muszynska G, Bretner M, Lefebvre O, Boguta M

Maf1 protein is a global negative regulator of RNA polymerase (Pol) III transcription conserved from yeast to man. We report that phosphorylation of Maf1 by casein kinase II (CK2), a highly evolutionarily conserved eukaryotic kinase, is required for efficient Pol III transcription. Both recombinant human and yeast CK2 were able to phosphorylate purified human or yeast Maf1, indicating that Maf1 ... [more]

Proc. Natl. Acad. Sci. U.S.A. Mar. 22, 2011; 108(12);4926-31 [Pubmed: 21383183]

Throughput

  • Low Throughput

Additional Notes

  • Supplementary figure S4

Curated By

  • BioGRID