BAIT

VMA4

H(+)-transporting V1 sector ATPase subunit E, L000002459, YOR332W
Subunit E of the V1 domain of the vacuolar H+-ATPase (V-ATPase); V-ATPase is an electrogenic proton pump found throughout the endomembrane system; V1 domain has eight subunits; required for the V1 domain to assemble onto the vacuolar membrane; protein abundance increases in response to DNA replication stress
GO Process (1)
GO Function (1)
GO Component (3)
Saccharomyces cerevisiae (S288c)
PREY

VPS25

VPL12, VPT25, ESCRT-II subunit protein VPS25, YJR102C
Component of the ESCRT-II complex; ESCRT-II is involved in ubiquitin-dependent sorting of proteins into the endosome
Saccharomyces cerevisiae (S288c)

Phenotypic Enhancement

A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

ESCRT-II coordinates the assembly of ESCRT-III filaments for cargo sorting and multivesicular body vesicle formation.

Teis D, Saksena S, Judson BL, Emr SD

The sequential action of five distinct endosomal-sorting complex required for transport (ESCRT) complexes is required for the lysosomal downregulation of cell surface receptors through the multivesicular body (MVB) pathway. On endosomes, the assembly of ESCRT-III is a highly ordered process. We show that the length of ESCRT-III (Snf7) oligomers controls the size of MVB vesicles and addresses how ESCRT-II regulates ... [more]

EMBO J. Mar. 03, 2010; 29(5);871-83 [Pubmed: 20134403]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: vesicle distribution (APO:0000302)

Additional Notes

  • Overexpression of SNF7 decreases the number of vesicles Vma4 backgound

Curated By

  • BioGRID