BAIT

KEG

KEEP ON GOING, T6I14.60, T6I14_60, AT5G13530
E3 ubiquitin-protein ligase KEG
Arabidopsis thaliana (Columbia)
PREY

EDR1

ATEDR1, ENHANCED DISEASE RESISTANCE 1, F22O13.20, F22O13_20, MAPKK KINASE EDR1, AT1G08720
serine/threonine-protein kinase EDR1
Arabidopsis thaliana (Columbia)

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

The Keep On Going (KEG) protein of Arabidopsis Recruits the Enhanced Disease Resistance 1 (EDR1) Protein to TGN/EE vesicles.

Gu Y, Innes RW

Loss-of-function mutations in the Arabidopsis thaliana ENHANCED DISEASE RESISTANCE 1 (EDR1) gene confer enhanced resistance to powdery mildew infection, enhanced senescence, and enhanced programmed cell death (PCD) under both abiotic and biotic stress conditions. All edr1-mediated phenotypes can be suppressed by a specific missense mutation (keg-4) in the KEEP ON GOING (KEG) gene, which encodes a multi-domain protein that includes ... [more]

Unknown Feb. 22, 2011; 0(0); [Pubmed: 21343429]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
EDR1 KEG
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
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Curated By

  • BioGRID