PFT1
Gene Ontology Biological Process
- defense response to fungus [IMP]
- jasmonic acid mediated signaling pathway [IMP]
- positive regulation of defense response [IMP]
- positive regulation of flower development [IMP]
- positive regulation of transcription, DNA-templated [IMP]
- red, far-red light phototransduction [IMP]
- regulation of flower development [IMP]
- response to far red light [IMP]
- response to red light [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- mediator complex [IDA]
- nucleus [IDA, ISM]
AT3G23230
Gene Ontology Biological Process
Gene Ontology Molecular Function
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
A High-Throughput Screening System for Arabidopsis Transcription Factors and Its Application to Med25-Dependent Transcriptional Regulation.
The activities of transcription factors (TFs) require interactions with specific DNA sequences and other regulatory proteins. To detect such interactions in Arabidopsis, we developed a high-throughput screening system with a Gateway-compatible Gal4-AD-TF library of 1589 Arabidopsis TFs, which can be easily screened by mating-based yeast-one-hybrid (Y1H) and yeast-two-hybrid (Y2H) methods. The efficiency of the system was validated by examining two ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| AT3G23230 PFT1 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| PFT1 AT3G23230 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID