BAIT
ROM1
SKC1, Rho family guanine nucleotide exchange factor ROM1, L000003181, YGR070W
GDP/GTP exchange protein (GEP) for Rho1p; mutations are synthetically lethal with mutations in rom2, which also encodes a GEP; ROM1 has a paralog, ROM2, that arose from the whole genome duplication
GO Process (2)
GO Function (1)
GO Component (1)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
LRG1
L000000953, YDL240W
GTPase-activating protein (GAP); contains Rho1p-specific GAP activity, interacting with activated forms of Rho1p; functions along with Sac7p as a negative regulator of the Pkc1p-mediated cell wall integrity signaling pathway; negative regulator of cell wall 1,3-beta-glucan biosynthesis; required for efficient cell fusion; contains a RhoGAP domain and three Lin-11-Isl1-Mec-3 (LIM) domains
GO Process (6)
GO Function (1)
GO Component (7)
Gene Ontology Biological Process
- cytogamy [IGI, IMP]
- negative regulation of Rho protein signal transduction [IGI, IMP]
- negative regulation of protein kinase C signaling [IGI, IMP]
- positive regulation of Rho GTPase activity [IDA]
- regulation of cell wall (1->3)-beta-D-glucan biosynthetic process [IGI]
- regulation of fungal-type cell wall organization [IGI, IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
Synthetic Rescue
A genetic interaction is inferred when mutations or deletions of one gene rescues the lethality or growth defect of a strain mutated or deleted for another gene.
Publication
Mechanisms for concentrating Rho1 during cytokinesis.
The small GTP-binding protein, Rho1/RhoA plays a central role in cytokinetic actomyosin ring (CAR) assembly and cytokinesis. Concentration of Rho proteins at the division site is a general feature of cytokinesis, yet the mechanisms for recruiting Rho to the division site for cytokinesis remain poorly understood. We find that budding yeast utilizes two mechanisms to concentrate Rho1 at the division ... [more]
Genes Dev. Apr. 01, 2009; 23(7);810-23 [Pubmed: 19339687]
Throughput
- Low Throughput
Ontology Terms
- phenotype: viability (APO:0000111)
Additional Notes
- deletion of Lrg1 rescues the lethality of a Cdc24/Rom1/Rom2 triple mutant
- genetic complex
Related interactions
Curated By
- BioGRID