BAIT
LRG1
L000000953, YDL240W
GTPase-activating protein (GAP); contains Rho1p-specific GAP activity, interacting with activated forms of Rho1p; functions along with Sac7p as a negative regulator of the Pkc1p-mediated cell wall integrity signaling pathway; negative regulator of cell wall 1,3-beta-glucan biosynthesis; required for efficient cell fusion; contains a RhoGAP domain and three Lin-11-Isl1-Mec-3 (LIM) domains
GO Process (6)
GO Function (1)
GO Component (7)
Gene Ontology Biological Process
- cytogamy [IGI, IMP]
- negative regulation of Rho protein signal transduction [IGI, IMP]
- negative regulation of protein kinase C signaling [IGI, IMP]
- positive regulation of Rho GTPase activity [IDA]
- regulation of cell wall (1->3)-beta-D-glucan biosynthetic process [IGI]
- regulation of fungal-type cell wall organization [IGI, IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
TUS1
SOP10, Rho family guanine nucleotide exchange factor TUS1, L000004619, L000004622, YLR425W
Guanine nucleotide exchange factor (GEF) that modulate Rho1p activity; involved in the cell integrity signaling pathway; interacts with Rgl1p; localization of Tus1p to the bed neck is regulated by Rgl1p; multicopy suppressor of tor2 mutation and ypk1 ypk2 double mutation; potential Cdc28p substrate
GO Process (2)
GO Function (1)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
Phenotypic Suppression
A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.
Publication
Mechanisms for concentrating Rho1 during cytokinesis.
The small GTP-binding protein, Rho1/RhoA plays a central role in cytokinetic actomyosin ring (CAR) assembly and cytokinesis. Concentration of Rho proteins at the division site is a general feature of cytokinesis, yet the mechanisms for recruiting Rho to the division site for cytokinesis remain poorly understood. We find that budding yeast utilizes two mechanisms to concentrate Rho1 at the division ... [more]
Genes Dev. Apr. 01, 2009; 23(7);810-23 [Pubmed: 19339687]
Throughput
- Low Throughput
Ontology Terms
- actin cytoskeleton morphology (APO:0000120)
Additional Notes
- Overexpression of Tus1 largely restores cytokinesis actomysin ring assembly in an lrg1/rom1/rom2/rho1/cdc24 mutant
- genetic complex
Curated By
- BioGRID