BAIT

NEO1

putative aminophospholipid-translocating P4-type ATPase NEO1, L000004112, YIL048W
Putative aminophospholipid translocase (flippase); involved in endocytosis, vacuolar biogenesis and Golgi to ER vesicle-mediated transport; localizes to endosomes and the Golgi apparatus
Saccharomyces cerevisiae (S288c)
PREY

VMA5

CSL5, VAT3, H(+)-transporting V1 sector ATPase subunit C, L000002460, YKL080W
Subunit C of the V1 peripheral membrane domain of V-ATPase; part of the electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane; the V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase) has eight subunits
GO Process (1)
GO Function (1)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Dosage Rescue

A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene.

Publication

Genome-Wide Analysis Reveals the Vacuolar pH-Stat of Saccharomyces cerevisiae.

Brett CL, Kallay L, Hua Z, Green R, Chyou A, Zhang Y, Graham TR, Donowitz M, Rao R

Protons, the smallest and most ubiquitous of ions, are central to physiological processes. Transmembrane proton gradients drive ATP synthesis, metabolite transport, receptor recycling and vesicle trafficking, while compartmental pH controls enzyme function. Despite this fundamental importance, the mechanisms underlying pH homeostasis are not entirely accounted for in any organelle or organism. We undertook a genome-wide survey of vacuole pH (pH(v)) ... [more]

PLoS ONE Mar. 23, 2011; 6(3);e17619 [Pubmed: 21423800]

Throughput

  • Low Throughput

Ontology Terms

  • heat sensitivity (APO:0000147)

Curated By

  • BioGRID