RRR1, SIR5, origin recognition complex subunit 2, L000001776, YBR060C
Subunit of the origin recognition complex (ORC); ORC directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; interacts with Spp1p and with trimethylated histone H3; phosphorylated by Cdc28p
Saccharomyces cerevisiae (S288c)


E1 ubiquitin-activating protein UBA1, L000002405, YKL210W
Ubiquitin activating enzyme (E1); involved in ubiquitin-mediated protein degradation and essential for viability; protein abundance increases in response to DNA replication stress
GO Process (1)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Phenotypic Suppression

A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.


ORC and the intra-S-phase checkpoint: a threshold regulates Rad53p activation in S phase.

Shimada K, Pasero P, Gasser SM

The intra-S-phase checkpoint in yeast responds to stalled replication forks by activating the ATM-like kinase Mec1 and the CHK2-related kinase Rad53, which in turn inhibit spindle elongation and late origin firing and lead to a stabilization of DNA polymerases at arrested forks. A mutation that destabilizes the second subunit of the Origin Recognition Complex, orc2-1, reduces the number of functional ... [more]

Genes Dev. Dec. 15, 2002; 16(24);3236-52 [Pubmed: 12502744]


  • Low Throughput

Ontology Terms

  • phenotype: protein/peptide modification (APO:0000131)

Additional Notes

  • rad53 phosphorylation in response to HU

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
Synthetic Rescue
Synthetic Rescue

A genetic interaction is inferred when mutations or deletions of one gene rescues the lethality or growth defect of a strain mutated or deleted for another gene.


Curated By

  • BioGRID