BAIT
ORC2
RRR1, SIR5, origin recognition complex subunit 2, L000001776, YBR060C
Subunit of the origin recognition complex (ORC); ORC directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; interacts with Spp1p and with trimethylated histone H3; phosphorylated by Cdc28p
GO Process (5)
GO Function (2)
GO Component (3)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
UBA1
E1 ubiquitin-activating protein UBA1, L000002405, YKL210W
Ubiquitin activating enzyme (E1); involved in ubiquitin-mediated protein degradation and essential for viability; protein abundance increases in response to DNA replication stress
GO Process (1)
GO Function (1)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Saccharomyces cerevisiae (S288c)
Phenotypic Suppression
A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.
Publication
ORC and the intra-S-phase checkpoint: a threshold regulates Rad53p activation in S phase.
The intra-S-phase checkpoint in yeast responds to stalled replication forks by activating the ATM-like kinase Mec1 and the CHK2-related kinase Rad53, which in turn inhibit spindle elongation and late origin firing and lead to a stabilization of DNA polymerases at arrested forks. A mutation that destabilizes the second subunit of the Origin Recognition Complex, orc2-1, reduces the number of functional ... [more]
Genes Dev. Dec. 15, 2002; 16(24);3236-52 [Pubmed: 12502744]
Throughput
- Low Throughput
Ontology Terms
- phenotype: protein/peptide modification (APO:0000131)
Additional Notes
- rad53 phosphorylation in response to HU
Related interactions
Curated By
- BioGRID