BAIT

AT1G61210

F11P17.7, F11P17_7
protein DWD hypersensitive to ABA 3
GO Process (2)
GO Function (1)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)
PREY

DDB1B

F7J7.40, F7J7_40, damaged DNA binding protein 1B, AT4G21100
DNA damage-binding protein 1b
GO Process (1)
GO Function (2)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

DWA3, an Arabidopsis DWD protein, acts as a negative regulator in ABA signal transduction.

Lee JH, Terzaghi W, Deng XW

DWD (DDB1 binding WD40) proteins have been reported as substrate receptors for cullin-RING ubiquitin ligase 4 complexes (CRL4), a family of E3 ligases. Abscisic acid (ABA) plays an important role in plant responses to abiotic stresses, such as drought and salinity. Upon screening T-DNA mutants of DWD genes for ABA responses we obtained several candidates that exhibited ABA-hypersensitivity, and one ... [more]

Plant Sci. Feb. 01, 2011; 180(2);352-7 [Pubmed: 21421380]

Throughput

  • Low Throughput

Curated By

  • BioGRID