Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.


RBP2-H1/JARID1B is a transcriptional regulator with a tumor suppressive potential in melanoma cells.

Roesch A, Mueller AM, Stempfl T, Moehle C, Landthaler M, Vogt T

The RBP2-H1/JARID1B nuclear protein belongs to the ARID family of DNA-binding proteins and is a potential tumor suppressor that is lost during melanoma development. As we have recently shown, one physiological function of RBP2-H1/JARID1B is to exert cell cycle control via maintenance of active retinoblastoma protein. We now add new evidence that RBP2-H1/JARID1B can also directly regulate gene transcription in ... [more]

Int. J. Cancer Mar. 01, 2008; 122(5);1047-57 [Pubmed: 17973255]


  • Low Throughput

Curated By

  • BioGRID