PREY

SMARCA4

BAF190, BAF190A, BRG1, MRD16, RTPS2, SNF2, SNF2L4, SNF2LB, SWI2, hSNF2b
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
GO Process (17)
GO Function (14)
GO Component (9)
Homo sapiens

Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Publication

A methylation-mediator complex in hormone signaling.

Xu W, Cho H, Kadam S, Banayo EM, Anderson S, Yates JR, Emerson BM, Evans RM

The recruitment of coactivators by nuclear hormone receptors (NRs) promotes transcription by subverting chromatin-mediated repression. Although the histone methylation enzyme CARM1 and an ATP-remodeling complex have been individually implicated in nuclear receptor-dependent transcription, neither a functional nor mechanistic linkage between these systems has been identified. In the process of purifying endogenous CARM1-interacting proteins, we identified an associated complex, nucleosomal methylation ... [more]

Genes Dev. Jan. 15, 2004; 18(2);144-56 [Pubmed: 14729568]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
CARM1 SMARCA4
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-
CARM1 SMARCA4
Co-purification
Co-purification

An interaction is inferred from the identification of two or more protein subunits in a purified protein complex, as obtained by classical biochemical fractionation or affinity purification and one or more additional fractionation steps.

Low-BioGRID
-
SMARCA4 CARM1
Far Western
Far Western

An interaction is detected between a protein immobilized on a membrane and a purified protein probe.

Low-BioGRID
-

Curated By

  • BioGRID