Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

The BRCT-domain containing protein PTIP links PAX2 to a histone H3, lysine 4 methyltransferase complex.

Patel SR, Kim D, Levitan I, Dressler GR

The MLL family of histone methyltransferases maintains active chromatin domains by methylating histone H3 on lysine 4 (H3K4). How MLL complexes recognize specific chromatin domains in a temporal and tissue-specific manner remains unclear. We show that the DNA-binding protein PAX2 promotes assembly of an H3K4 methyltransferase complex through the ubiquitously expressed nuclear factor PTIP (pax transcription activation domain interacting protein). ... [more]

Dev. Cell Oct. 01, 2007; 13(4);580-92 [Pubmed: 17925232]

Throughput

  • Low Throughput

Curated By

  • BioGRID