BAIT
SLI15
L000003320, YBR156C
Subunit of the conserved chromosomal passenger complex (CPC); complex regulates kinetochore-microtubule attachments, activation of the spindle tension checkpoint, and mitotic spindle disassembly; other complex members are Ipl1p, Bir1p, and Nbl1p
GO Process (3)
GO Function (1)
GO Component (4)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
SKP1
MGO1, SCF ubiquitin ligase subunit SKP1, CBF3D, L000001910, YDR328C
Evolutionarily conserved kinetochore protein; part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase; protein abundance increases in response to DNA replication stress
GO Process (12)
GO Function (4)
GO Component (6)
Gene Ontology Biological Process
- G1/S transition of mitotic cell cycle [IMP]
- G2/M transition of mitotic cell cycle [IMP]
- SCF-dependent proteasomal ubiquitin-dependent protein catabolic process [IDA]
- exit from mitosis [IMP]
- kinetochore assembly [IDA, IMP]
- protein complex assembly [IDA]
- protein neddylation [IGI, IMP]
- protein ubiquitination involved in ubiquitin-dependent protein catabolic process [IDA]
- regulation of exit from mitosis [IMP, IPI]
- regulation of protein complex assembly [IPI]
- septin ring assembly [IMP]
- vacuolar acidification [IGI, IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Systematic exploration of essential yeast gene function with temperature-sensitive mutants.
Conditional temperature-sensitive (ts) mutations are valuable reagents for studying essential genes in the yeast Saccharomyces cerevisiae. We constructed 787 ts strains, covering 497 (∼45%) of the 1,101 essential yeast genes, with ∼30% of the genes represented by multiple alleles. All of the alleles are integrated into their native genomic locus in the S288C common reference strain and are linked to ... [more]
Unknown Mar. 27, 2011; 0(0); [Pubmed: 21441928]
Throughput
- High Throughput
Curated By
- BioGRID