BAIT

ERG8

phosphomevalonate kinase, L000000574, YMR220W
Phosphomevalonate kinase; an essential cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate
Saccharomyces cerevisiae (S288c)
PREY

ERG9

bifunctional farnesyl-diphosphate farnesyltransferase/squalene synthase, L000000575, YHR190W
Farnesyl-diphosphate farnesyl transferase (squalene synthase); joins two farnesyl pyrophosphate moieties to form squalene in the sterol biosynthesis pathway
GO Process (1)
GO Function (2)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Phenotypic Enhancement

A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

Linking Genotype and Phenotype of Saccharomyces cerevisiae Strains Reveals Metabolic Engineering Targets and Leads to Triterpene Hyper-Producers.

Madsen KM, Udatha GD, Semba S, Otero JM, Koetter P, Nielsen J, Ebizuka Y, Kushiro T, Panagiotou G

Metabolic engineering is an attractive approach in order to improve the microbial production of drugs. Triterpenes is a chemically diverse class of compounds and many among them are of interest from a human health perspective. A systematic experimental or computational survey of all feasible gene modifications to determine the genotype yielding the optimal triterpene production phenotype is a laborious and ... [more]

PLoS ONE Mar. 30, 2011; 6(3);e14763 [Pubmed: 21445244]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: chemical compound accumulation (APO:0000095)

Additional Notes

  • genetic complex
  • overexpression of both genes results in a synergistic increase in the production of ergosterol
  • overexpression of erg8, erg9 and hfa1 causes a synthetic increase in ergosterol production over that seen in the double mutants

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
ERG9 ERG8
Positive Genetic
Positive Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a less severe fitness defect than expected under a given condition. This term is reserved for high or low throughput studies with scores.

High0.1699BioGRID
1873732

Curated By

  • BioGRID