BAIT
RHO3
Rho family GTPase RHO3, L000001632, YIL118W
Non-essential small GTPase of the Rho/Rac family of Ras-like proteins; involved in the establishment of cell polarity; GTPase activity positively regulated by the GTPase activating protein (GAP) Rgd1p
GO Process (3)
GO Function (2)
GO Component (3)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
BNI1
PPF3, SHE5, formin BNI1, L000000190, YNL271C
Formin; polarisome component; nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1
GO Process (9)
GO Function (1)
GO Component (8)
Gene Ontology Biological Process
- actin filament bundle assembly [IGI, IMP]
- actin nucleation [IDA]
- actomyosin contractile ring actin filament bundle assembly [IMP]
- barbed-end actin filament capping [IDA]
- budding cell apical bud growth [IGI, IMP]
- establishment of mitotic spindle orientation [IMP]
- formin-nucleated actin cable assembly [IDA, IGI, IMP]
- positive regulation of actin cytoskeleton reorganization [IGI, IMP]
- regulation of protein localization [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
Synthetic Rescue
A genetic interaction is inferred when mutations or deletions of one gene rescues the lethality or growth defect of a strain mutated or deleted for another gene.
Publication
Formin-dependent actin assembly is regulated by distinct modes of Rho signaling in yeast.
Formins are actin filament nucleators regulated by Rho-GTPases. In budding yeast, the formins Bni1p and Bnr1p direct the assembly of actin cables, which guide polarized secretion and growth. From the six yeast Rho proteins (Cdc42p and Rho1-5p), we have determined that four participate in the regulation of formin activity. We show that the essential function of Rho3p and Rho4p is ... [more]
J. Cell Biol. Jun. 23, 2003; 161(6);1081-92 [Pubmed: 12810699]
Throughput
- Low Throughput
Ontology Terms
- phenotype: viability (APO:0000111)
Additional Notes
- genetic complex
- mutation of bni1 rescues growth in a bnr1/rho3 mutant
- mutation of bni1 rescues growth in a rho3/rho4 mutant
Related interactions
Curated By
- BioGRID