BAIT
GIS4
L000004864, YML006C
CAAX box containing protein of unknown function; proposed to be involved in the RAS/cAMP signaling pathway
GO Process (2)
GO Function (0)
GO Component (1)
Gene Ontology Biological Process
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
GIS1
histone demethylase GIS1, L000003351, YDR096W
Histone demethylase and transcription factor; regulates genes during nutrient limitation; activity modulated by proteasome-mediated proteolysis; has JmjC and JmjN domain in N-terminus that interact, promoting stability and proper transcriptional activity; contains two transactivating domains downstream of Jmj domains and a C-terminal DNA binding domain; relocalizes to the cytosol in response to hypoxia; GIS1 has a paralog, RPH1, that arose from the whole genome duplication
GO Process (6)
GO Function (5)
GO Component (3)
Gene Ontology Biological Process
- chronological cell aging [IMP]
- histone demethylation [IMP]
- maintenance of stationary phase in response to starvation [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IMP]
- regulation of phospholipid biosynthetic process [IMP]
Gene Ontology Molecular Function- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [IDA, IMP]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IC]
- histone demethylase activity (H3-K36 specific) [IMP]
- sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [IDA, IMP]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IC]
- histone demethylase activity (H3-K36 specific) [IMP]
- sequence-specific DNA binding [IDA]
Saccharomyces cerevisiae (S288c)
Dosage Rescue
A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene.
Publication
Yeast genes GIS1-4: multicopy suppressors of the Gal- phenotype of snf1 mig1 srb8/10/11 cells.
Cyclin C and the cyclin C-dependent protein kinase are associated with the RNA polymerase II Mediator complex, which regulates initiation of transcription in response to signals from activators and repressors bound to upstream promoter elements. Disruption of the corresponding genes, SRB11 and SRB10, in budding yeast causes a reduction in expression of the GAL genes, which is particularly pronounced in ... [more]
Mol. Gen. Genet. Dec. 01, 1999; 262(4);589-99 [Pubmed: 10628841]
Throughput
- Low Throughput
Ontology Terms
- phenotype: utilization of carbon source (APO:0000098)
- phenotype: vegetative growth (APO:0000106)
- phenotype: heat sensitivity (APO:0000147)
Additional Notes
- Overexpression of Gis1 can rescue growth in an snf1/mig1/gis4 triple mutant
- Overexpression of PDE2 can rescue growth in an snf1/mig1/gis4 triple mutant
- genetic complex
Curated By
- BioGRID