BAIT

DDB1A

C6L9.100, C6L9_100, damaged DNA binding protein 1A, AT4G05420
DNA damage-binding protein 1a
GO Process (2)
GO Function (2)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)
PREY

CLF

CURLY LEAF, F26B6.3, F26B6_3, ICU1, INCURVATA 1, SDG1, SET1, SETDOMAIN 1, SETDOMAIN GROUP 1, AT2G23380
histone-lysine N-methyltransferase CLF
Arabidopsis thaliana (Columbia)

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

MSI4/FVE interacts with CUL4-DDB1 and a PRC2-like complex to control epigenetic regulation of flowering time in Arabidopsis.

Pazhouhandeh M, Molinier J, Berr A, Genschik P

Flowering at the right time is crucial to ensure successful plant reproduction and seed yield and is dependent on both environmental and endogenous parameters. Among the different pathways that impinge on flowering, the autonomous pathway promotes floral transition independently of day length through the repression of the central flowering repressor flowering locus C (FLC). FLC blocks floral transition by repressing ... [more]

Proc. Natl. Acad. Sci. U.S.A. Feb. 22, 2011; 108(8);3430-5 [Pubmed: 21282611]

Throughput

  • Low Throughput

Curated By

  • BioGRID