BAIT

SKO1

ACR1, L000001909, YNL167C
Basic leucine zipper transcription factor of the ATF/CREB family; forms a complex with Tup1p and Cyc8p to both activate and repress transcription; cytosolic and nuclear protein involved in osmotic and oxidative stress responses
Saccharomyces cerevisiae (S288c)
PREY

SMP1

L000004146, YBR182C
MADS-box transcription factor involved in osmotic stress response; SMP1 has a paralog, RLM1, that arose from the whole genome duplication
Saccharomyces cerevisiae (S288c)

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Prediction of putative protein interactions through evolutionary analysis of osmotic stress response in the model yeast Saccharomyces cerevisae.

Thorne TW, Ho HL, Huvet M, Haynes K, Stumpf MP

The osmotic stress response signalling pathway of the model yeast Saccharomyces cerevisae is crucial for the survival of cells under osmotic stress, and is preserved to varying degrees in other related fungal species. We apply a method for inference of ancestral states of characteristics over a phylogeny to 17 fungal species to infer the maximum likelihood estimate of presence or ... [more]

Fungal Genet. Biol. May. 01, 2011; 48(5);504-11 [Pubmed: 21193057]

Throughput

  • Low Throughput

Additional Notes

  • Directionality not explicit in paper

Curated By

  • BioGRID