BAIT

NF2

merlin, RP23-280J3.6
neurofibromatosis 2
GO Process (25)
GO Function (4)
GO Component (18)
Mus musculus
PREY

CTNNB1

Bfc, Catnb, Mesc
catenin (cadherin associated protein), beta 1
GO Process (137)
GO Function (14)
GO Component (29)

Gene Ontology Biological Process

Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

NF2 deficiency promotes tumorigenesis and metastasis by destabilizing adherens junctions.

Lallemand D, Curto M, Saotome I, Giovannini M, McClatchey AI

Mutation of the Neurofibromatosis 2 (NF2) tumor suppressor gene leads to cancer development in humans and mice. Recent studies suggest that Nf2 loss also contributes to tumor metastasis. The Nf2-encoded protein, merlin, is related to the ERM (ezrin, radixin, and moesin) family of membrane:cytoskeleton-associated proteins. However, the cellular mechanism whereby merlin controls cell proliferation from this location is not known. ... [more]

Genes Dev. May. 01, 2003; 17(9);1090-100 [Pubmed: 12695331]

Throughput

  • Low Throughput

Curated By

  • BioGRID