Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.


USF1 recruits histone modification complexes and is critical for maintenance of a chromatin barrier.

Huang S, Li X, Yusufzai TM, Qiu Y, Felsenfeld G

The insulator element at the 5' end of the chicken beta-globin locus acts as a barrier, protecting transgenes against silencing effects of adjacent heterochromatin. We showed earlier that the transcription factor USF1 binds within the insulator and that this site is important for generating in adjacent nucleosomes histone modifications associated with active chromatin and, by inference, with barrier function. To ... [more]

Mol. Cell. Biol. Nov. 01, 2007; 27(22);7991-8002 [Pubmed: 17846119]


  • Low Throughput

Curated By

  • BioGRID