BAIT

KAR2

GRP78, Hsp70 family ATPase KAR2, BIP, L000000887, YJL034W
ATPase involved in protein import into the ER; also acts as a chaperone to mediate protein folding in the ER and may play a role in ER export of soluble proteins; regulates the unfolded protein response via interaction with Ire1p
Saccharomyces cerevisiae (S288c)
PREY

SLG1

HCS77, WSC1, L000003080, YOR008C
Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response
Saccharomyces cerevisiae (S288c)

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Evasion of endoplasmic reticulum surveillance makes Wsc1p an obligate substrate of Golgi quality control.

Wang S, Ng DT

In the endoplasmic reticulum (ER), most newly synthesized proteins are retained by quality control mechanisms until folded. Misfolded molecules are sorted to ER-associated degradation (ERAD) pathways for disposal. Reports of mutant proteins degraded in the vacuole/lysosome suggested an independent Golgi-based mechanism also at work. Although little is understood of the post-ER pathway, the growing number of variants using it suggests ... [more]

Mol. Biol. Cell Apr. 01, 2010; 21(7);1153-65 [Pubmed: 20130083]

Throughput

  • Low Throughput

Additional Notes

  • misfolded Wsc1 fused with an ERAD determinant binds Kar2

Curated By

  • BioGRID