BAIT

LAC1

DGT1, sphingosine N-acyltransferase LAC1, YKL008C
Ceramide synthase component; involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lag1p; LAC1 has a paralog, LAG1, that arose from the whole genome duplication
GO Process (2)
GO Function (1)
GO Component (3)
Saccharomyces cerevisiae (S288c)
PREY

SLC1

1-acylglycerol-3-phosphate O-acyltransferase SLC1, L000001914, YDL052C
1-acyl-sn-glycerol-3-phosphate acyltransferase; catalyzes the acylation of lysophosphatidic acid to form phosphatidic acid, a key intermediate in lipid metabolism; enzymatic activity detected in lipid particles and microsomes
GO Process (1)
GO Function (1)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Synthetic Rescue

A genetic interaction is inferred when mutations or deletions of one gene rescues the lethality or growth defect of a strain mutated or deleted for another gene.

Publication

Yeast cells lacking all known ceramide synthases continue to make complex sphingolipids and to incorporate ceramides into glycosylphosphatidylinositol (GPI) anchors.

Vionnet C, Roubaty C, Ejsing CS, Knudsen J, Conzelmann A

In yeast, the inositolphosphorylceramides mostly contain C26:0 fatty acids. Inositolphosphorylceramides were considered to be important for viability because the inositolphosphorylceramide synthase AUR1 is essential. However, lcb1Δ cells, unable to make sphingoid bases and inositolphosphorylceramides, are viable if they harbor SLC1-1, a gain of function mutation in the 1-acyl-glycerol-3-phosphate acyltransferase SLC1. SLC1-1 allows the incorporation of C26:0 fatty acids into phosphatidylinositol ... [more]

J. Biol. Chem. Feb. 25, 2011; 286(8);6769-79 [Pubmed: 21173150]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: viability (APO:0000111)

Additional Notes

  • Expression of a gain of function form of SLC1 rescues the growth of YPC1/YDC1/LAG1/LAC1 quadruple mutants
  • genetic complex

Curated By

  • BioGRID