Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Assessment of splice variant-specific functions of desmocollin 1 in the skin.

Cheng X, Mihindukulasuriya K, Den Z, Kowalczyk AP, Calkins CC, Ishiko A, Shimizu A, Koch PJ

Desmocollin 1 (Dsc1) is part of a desmosomal cell adhesion receptor formed in terminally differentiating keratinocytes of stratified epithelia. The dsc1 gene encodes two proteins (Dsc1a and Dsc1b) that differ only with respect to their COOH-terminal cytoplasmic amino acid sequences. On the basis of in vitro experiments, it is thought that the Dsc1a variant is essential for assembly of the ... [more]

Mol. Cell. Biol. Jan. 01, 2004; 24(1);154-63 [Pubmed: 14673151]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
PKP1 DSC1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Marcon E (2014)	High	11.8299	BioGRID	2951604

Curated By

BioGRID

Interaction Summary

PKP1

Gene Ontology Biological Process

Gene Ontology Molecular Function

intermediate filament binding [NAS]lamin binding [IDA]protein binding [IPI]signal transducer activity [TAS]structural constituent of epidermis [NAS]

Gene Ontology Cellular Component

DSC1