BAIT

PCP2

L7, Pcp-2
Purkinje cell protein 2 (L7)
GO Process (2)
GO Function (1)
GO Component (1)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Mus musculus
PREY

CTNNB1

Bfc, Catnb, Mesc
catenin (cadherin associated protein), beta 1
GO Process (137)
GO Function (14)
GO Component (29)

Gene Ontology Biological Process

Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Intracellular substrates of brain-enriched receptor protein tyrosine phosphatase rho (RPTPrho/PTPRT).

Besco JA, Hooft van Huijsduijnen R, Frostholm A, Rotter A

Receptor protein tyrosine phosphatase rho (RPTPrho/PTPRT) is a transmembrane protein that is highly expressed in the developing and adult central nervous system. It is a member of the RPTP R2B subfamily, which includes PTPkappa, PTPmu and PCP-2. Glutathione-S-transferase (GST) pulldown assays were used to show that RPTPrho interacts with several adherens junctional proteins in brain, including E-cadherin, N-cadherin, VE-cadherin (cadherin-5), ... [more]

Brain Res. Oct. 20, 2006; 1116(1);50-7 [Pubmed: 16973135]

Throughput

  • Low Throughput

Curated By

  • BioGRID