parkin RBR E3 ubiquitin protein ligase
GO Process (58)
GO Function (22)
GO Component (12)

Gene Ontology Biological Process

Homo sapiens


SYN1a, SYN1b, SYNI, RP1-230G1.2
synapsin I
GO Process (3)
GO Function (4)
GO Component (1)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.


Ubiquitination of a new form of alpha-synuclein by parkin from human brain: implications for Parkinson's disease.

Shimura H, Schlossmacher MG, Hattori N, Frosch MP, Trockenbacher A, Schneider R, Mizuno Y, Kosik KS, Selkoe DJ

Parkinson's disease (PD) is a common neurodegenerative disorder characterized by the progressive accumulation in selected neurons of protein inclusions containing alpha-synuclein and ubiquitin. Rare inherited forms of PD are caused by autosomal dominant mutations in alpha-synuclein or by autosomal recessive mutations in parkin, an E3 ubiquitin ligase. We hypothesized that these two gene products interact functionally, namely, that parkin ubiquitinates ... [more]

Science Jul. 13, 2001; 293(5528);263-9 [Pubmed: 11431533]


  • Low Throughput

Curated By

  • BioGRID