BAIT

LRO1

phospholipid:diacylglycerol acyltransferase, S000007453, YNR008W
Acyltransferase that catalyzes diacylglycerol esterification; one of several acyltransferases that contribute to triglyceride synthesis; Lro1p and Dga1p can O-acylate ceramides; putative homolog of human lecithin cholesterol acyltransferase
GO Process (3)
GO Function (1)
GO Component (2)
Saccharomyces cerevisiae (S288c)
PREY

PAH1

SMP2, phosphatidate phosphatase PAH1, L000001934, YMR165C
Mg2+-dependent phosphatidate (PA) phosphatase; dephosphorylates PA to yield diacylglycerol; responsible for de novo lipid synthesis and formation of lipid droplets; phosphorylation by Pho80p-Pho85p decreases catalytic activity and alters Pah1p localization and abundance; phosphorylation by protein kinase A decreases catalytic efficiency; dephosphorylation by Nem1p-Spo7p anchors Pah1p to the membrane increasing substrate catalysis; homologous to mammalian lipins 1 and 2
Saccharomyces cerevisiae (S288c)

Phenotypic Enhancement

A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

The yeast lipin orthologue Pah1p is important for biogenesis of lipid droplets.

Adeyo O, Horn PJ, Lee S, Binns DD, Chandrahas A, Chapman KD, Goodman JM

Lipins are phosphatidate phosphatases that generate diacylglycerol (DAG). In this study, we report that yeast lipin, Pah1p, controls the formation of cytosolic lipid droplets. Disruption of PAH1 resulted in a 63% decrease in droplet number, although total neutral lipid levels did not change. This was accompanied by an accumulation of neutral lipids in the endoplasmic reticulum (ER). The droplet biogenesis ... [more]

J. Cell Biol. Mar. 21, 2011; 192(6);1043-55 [Pubmed: 21422231]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: lipid particle morphology (APO:0000242)

Additional Notes

  • deletion of pah1 in an dga1/lro1 double mutant decreases the formation of lipid droplets
  • genetic complex

Curated By

  • BioGRID